Research on genetic improvement and new breeding techniques of A. sinensis at Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences: Based on the comparison of breeding and labeling individual mixed growth of previous generation breeding and breeding, the growth traits of individual individuals with different families were studied. Determination and comparative analysis. According to the principles of quantitative genetics, based on the pedigree, individual's growth performance and kinship, the BLUP method was used to determine the design scheme for the breeding of the second generation of larval pedigree breeding and the parental control of the control family. Paired breeding of 90 breeding families and 20 control families was conducted, and 81 F2 breeding families and 19 control families were successfully established. The progenies of the second generation and 19 control families of the 81 herring families were subjected to isolation and cultivation of the internal family lines, individual marker and mixed pond culture after labeling under substantially the same conditions. The final measurement and analysis and comparison of growth traits of second-generation marker individuals in pedigree breeding were conducted; in the Yunnan, Jiangsu, and Shandong provinces, a comparative trial of family breeding (second generation) and general breeding of Jian’an was conducted. It reaches 150 acres. It laid the foundation for the promotion and demonstration of the later implementation of the project. The third is the selection of new high-yielding and high-quality shrimp: Genetic diversity of six geographical populations of Macrobrachium nipponense was analyzed. The full-length ITS-1 sequence of Macrobrachium nipponense with a length of 1763 bp was determined for the first time, which is the longest reported ITS-1 sequence so far. At the same time, 76 SNP loci were screened. The microsatellite sequence of Macrobrachium nipponense was screened by magnetic bead enrichment method and 95 positive clones were obtained. 78 clones contained microsatellite sequences. Based on the analysis of flanking sequences, 40 pairs of microsatellite primers were designed. After screening by PCR, 32 pairs of target fragments could be amplified, of which 20 pairs were polymorphic in the population. One good hybridization combination was screened; two shrimp breeding materials were selected. Research on the breeding of green prawn: The establishment of artificial propagation, hybridization and individual marker techniques, the evaluation of breeding value of green prawn breeding BLUP, safe and effective anaesthesia technology, etc. A breeding program was established, 35 families were established, and the first generation breeding was performed; the growth rate was increased by 5% and the survival rate was increased by 10%. A total of 100 concrete tanks for family breeding were established, totalling 1,500 square meters.
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